Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper i proceeding, 2014
Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.
Diagnostics
Light sheet microscopy
Single Particle Tracking
Lab-on-a-chip
Författare
Hendrik Deschout
K. Raemdonck
S. Stremersch
P. Maoddi
G. Mernier
P. Renaud
S. Jiguet
A. Hendrix
M. Bracke
R. Van den Broecke
Magnus Röding
Göteborgs universitet
SuMo Biomaterials
Chalmers, Matematiska vetenskaper, matematisk statistik
Mats Rudemo
Chalmers, Matematiska vetenskaper, matematisk statistik
Göteborgs universitet
J. Demeester
S. De Smedt
F. Strubbe
K. Neyts
Kevin Braeckmans
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013
Vol. 3 2010-2012
978-16-32-66624-6 (ISBN)
Ämneskategorier (SSIF 2011)
Biokemi och molekylärbiologi
ISBN
978-16-32-66624-6