Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper in proceeding, 2014
Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.
Diagnostics
Light sheet microscopy
Single Particle Tracking
Lab-on-a-chip
Author
Hendrik Deschout
K. Raemdonck
S. Stremersch
P. Maoddi
G. Mernier
P. Renaud
S. Jiguet
A. Hendrix
M. Bracke
R. Van den Broecke
Magnus Röding
University of Gothenburg
SuMo Biomaterials
Chalmers, Mathematical Sciences, Mathematical Statistics
Mats Rudemo
Chalmers, Mathematical Sciences, Mathematical Statistics
University of Gothenburg
J. Demeester
S. De Smedt
F. Strubbe
K. Neyts
Kevin Braeckmans
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013
Vol. 3 2010-2012
978-16-32-66624-6 (ISBN)
Subject Categories (SSIF 2011)
Biochemistry and Molecular Biology
ISBN
978-16-32-66624-6