Nanosized fibers' effect on adult human articular chondrocytes behavior.
Artikel i vetenskaplig tidskrift, 2013
Tissue engineering with chondrogenic cell based therapies is an expanding field with the intention of treating cartilage defects. It has been suggested that scaffolds used in cartilage tissue engineering influence cellular behavior and thus the long-term clinical outcome. The objective of this study was to assess whether chondrocyte attachment, proliferation and post-expansion re-differentiation could be influenced by the size of the fibers presented to the cells in a scaffold. Polylactic acid (PLA) scaffolds with different fiber morphologies were produced, i.e. microfiber (MS) scaffolds as well as nanofiber-coated microfiber scaffold (NMS). Adult human articular chondrocytes were cultured in the scaffolds in vitro up to 28 days, and the resulting constructs were assessed histologically, immunohistochemically, and biochemically. Attachment of cells and serum proteins to the scaffolds was affected by the architecture. The results point toward nano-patterning onto the microfibers influencing proliferation of the chondrocytes, and the overall 3D environment having a greater influence on the re-differentiation. In the efforts of finding the optimal scaffold for cartilage tissue engineering, studies as the current contribute to the knowledge of how to affect and control chondrocytes behavior.
ultrastructure
Immunohistochemistry
Cell Shape
Cell Count
Articular
Tissue Scaffolds
Nanofibers
Chondrocytes
Cartilage
Particle Size
cytology
cytology
Humans
chemistry
Cell Adhesion
Adult
Chondrogenesis
Cell Differentiation
ultrastructure
Cell Proliferation
Författare
Hanna Stenhamre
Chalmers, Kemi- och bioteknik, Polymerteknologi
Anna Thorvaldsson
Chalmers, Kemi- och bioteknik, Polymerteknologi
Lars Enochson
Göteborgs universitet
Pernilla Walkenström
Swerea IVF AB
Anders Lindahl
Göteborgs universitet
Mats Brittberg
Göteborgs universitet
Paul Gatenholm
Chalmers, Kemi- och bioteknik, Polymerteknologi
Materials science & engineering. C, Materials for biological applications
1873-0191 (ISSN)
Vol. 33 3 1539-1545Ämneskategorier (SSIF 2011)
Materialteknik
Polymerteknologi
DOI
10.1016/j.msec.2012.12.059
PubMed
23827606