Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
Artikel i vetenskaplig tidskrift, 2014
Background: Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods: In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results: We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion: Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.
mineralization
human mesenchymal stem cells
implantable materials
cell proliferation
colloidal lithography
osteogenic differentiation
nanotopography
Författare
Giuseppe Maria de Peppo
Göteborgs universitet
Hossein Agheli
Göteborgs universitet
Camilla Karlsson
Göteborgs universitet
Karin Ekström
Göteborgs universitet
H. Brisby
Göteborgs universitet
Maria Lennerås
Göteborgs universitet
Stefan Gustafsson
Chalmers, Teknisk fysik, Eva Olsson Group
SuMo Biomaterials
Peter Sjövall
Chalmers, Teknisk fysik, Biologisk fysik
Anna Johansson
Göteborgs universitet
Eva Olsson
Chalmers, Teknisk fysik, Eva Olsson Group
Jukka Lausmaa
BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy
SP Technical Research Institute of Sweden
Peter Thomsen
Göteborgs universitet
Sarunas Petronis
BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy
SP Technical Research Institute of Sweden
International journal of nanomedicine
1176-9114 (ISSN) 1178-2013 (eISSN)
Vol. 9 1 2499-2515Ämneskategorier (SSIF 2011)
Biomaterialvetenskap
DOI
10.2147/ijn.s58805