Electrospun nanofibrous cellulose scaffolds with controlled microarchitecture
Artikel i vetenskaplig tidskrift, 2014
Introducing porosity in electrospun scaffolds is critical to improve cell penetration and nutrient diffusion for tissue engineering. Nanofibrous cellulose scaffolds were prepared by electrospinning cellulose acetate (CA) followed by saponification to regenerate cellulose. Using a computer-assisted design approach, scaffolds underwent laser ablation resulting in pores with diameters between 50 and 300 mu m. without damaging or modifying the surrounding scaffold area. A new mineralization method was employed in conjunction with microablation using commercial phosphate buffered saline (PBS) to soak carboxymethylcellulose surface-modified electrospun scaffolds. The resulting crystals within the scaffold on the interior of the pore had a calcium to phosphate ratio of 1.56, similar to hydroxyapatite. It was observed that porosity of the cellulose scaffolds enhanced osteoblast cell attachment at the edge of the pores, while mineralization enhanced overall cell density.
TISSUE ENGINEERING APPLICATIONS
Cellulose
ARCHITECTURE
Porosity
ACCOUNTS OF CHEMICAL RESEARCH
CALCIUM-PHOSPHATE
Hydroxyapatite
1975
P273
DESIGN
PEPTIDES
Scaffold
AFFINITY MEMBRANE
SNER AS
V8
Bone regeneration
HYDROXYAPATITE
SURFACE
Laser
ADHESION
CONSTRUCTS
Författare
K. Rodriguez
Virginia Polytechnic Institute and State University
Johan Sundberg
Chalmers, Kemi- och bioteknik, Polymerteknologi
Paul Gatenholm
Chalmers, Kemi- och bioteknik, Polymerteknologi
S. Renneckar
Virginia Polytechnic Institute and State University
Carbohydrate Polymers
0144-8617 (ISSN)
Vol. 100 143-149Ämneskategorier (SSIF 2011)
Polymerteknologi
DOI
10.1016/j.carbpol.2012.12.037