Fungal Pretreatment of Oil Palm Empty Fruit Bunch: Effect of Manganese and Nitrogen

Artikel i vetenskaplig tidskrift, 2013

Oil palm empty fruit bunch (OPEFB) was biologically pretreated using Pleurotus floridanus LIPIMC996 supplemented with various concentrations of manganese and nitrog en and incubated for 35 days at 30°C, and enzyme ac tivities of manganese peroxidase (MnP) and laccase were examine d. When OPEFB was supplemented with manganese, the highest lignin reduction was achieved up to 25.0±5. 6% at the addition of 200 μg Mn 2+ /g OPEFB.In addition, Pleurotus floridanus LIPIMC996 grew best on OPEFB supplemented with 800 μg Mn 2+ /g OPEFB. When OPEFB was supplemented with nitrogen, the highest lignin redu ction was achieved up to 27.2± 3.5% at the addition of 20 mM nitrogen. The best growth of Pleurotus floridanus was also achieved with the addition of 20 mM nitrog en. The addition of nitrogen and manganese on OPEFB did not signific antly affect the activity of MnP and laccase.