Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88
Artikel i vetenskaplig tidskrift, 2011
The filamentous fungus Aspergillus niger exhibits great diversity in its phenotype. It is found globally, both as marine and terrestrial strains, produces both organic acids and hydrolytic enzymes in high amounts, and some isolates exhibit pathogenicity. Although the genome of an industrial enzyme-producing A. niger strain (CBS 513.88) has already been sequenced, the versatility and diversity of this species compel additional exploration. We therefore undertook wholegenome sequencing of the acidogenic A. niger wild-type strain (ATCC 1015) and produced a genome sequence of very high quality. Only 15 gaps are present in the sequence, and half the telomeric regions have been elucidated. Moreover, sequence information from ATCC 1015 was used to improve the genome sequence of CBS 513.88. Chromosome-level comparisons uncovered several genome rearrangements, deletions, a clear case of strain-specific horizontal gene transfer, and identification of 0.8 Mb of novel sequence. Single nucleotide polymorphisms per kilobase (SNPs/kb) between the two strains were found to be exceptionally high (average: 7.8, maximum: 160 SNPs/kb). High variation within the species was confirmed with exo-metabolite profiling and phylogenetics. Detailed lists of alleles were generated, and genotypic differences were observed to accumulate in metabolic pathways essential to acid production and protein synthesis. A transcriptome analysis supported up-regulation of genes associated with biosynthesis of amino acids that are abundant in glucoamylase A, tRNA-synthases, and protein transporters in the protein producing CBS 513.88 strain. Our results and data sets from this integrative systems biology analysis resulted in a snapshot of fungal evolution and will support further optimization of cell factories based on filamentous fungi
Base Sequence
Gene Transfer
Genome
Gene Expression Profiling
Computational Biology
Gene Rearrangement
Genetic Variation
Molecular
Horizontal
Evolution
Författare
M. R. Andersen
Danmarks Tekniske Universitet
Margarita Salazar Pena
Chalmers University of Technology
Danmarks Tekniske Universitet
P. J. Schaap
Wageningen University and Research Centre
P. J. I. Van De Vondervoort
DSM Biotechnology Center
D. Culley
Pacific Northwest National Laboratory
J. Thykaer
Danmarks Tekniske Universitet
J. C. Frisvad
Danmarks Tekniske Universitet
K. F. Nielsen
Danmarks Tekniske Universitet
R. Albang
Biomax Informatics AG
K. Albermann
Biomax Informatics AG
R. M. Berka
Novozymes Biotech, Inc.
G. H. Braus
Universitat Gottingen
S. A. Braus-Stromeyer
Universitat Gottingen
L. M. Corrochano
Universidad de Sevilla
Z. Dai
Pacific Northwest National Laboratory
P. W. M. Van Dijck
DSM Nutritional Products
G. Hofmann
Novozymes AS
L. L. Lasure
Pacific Northwest National Laboratory
J. K. Magnuson
Pacific Northwest National Laboratory
H. Menke
DSM Biotechnology Center
M. Meijer
CBS Fungal Biodiversity Center CBS - KNAW
S. L. Meijer
Danmarks Tekniske Universitet
J. B. Nielsen
Danmarks Tekniske Universitet
M. L. Nielsen
Danmarks Tekniske Universitet
A. J. J. Van Ooyen
DSM Biotechnology Center
H. J. Pel
DSM Biotechnology Center
L. Poulsen
Danmarks Tekniske Universitet
R. A. Samson
CBS Fungal Biodiversity Center CBS - KNAW
H. Stam
DSM Biotechnology Center
A. Tsang
Universite Concordia
J. M. Van Den Brink
Chr. Hansen AS
A. Atkins
DOE Joint Genome Institute
A. Aerts
DOE Joint Genome Institute
H. Shapiro
DOE Joint Genome Institute
J. Pangilinan
DOE Joint Genome Institute
A. Salamov
DOE Joint Genome Institute
Y. Lou
DOE Joint Genome Institute
E. Lindquist
DOE Joint Genome Institute
S. Lucas
DOE Joint Genome Institute
J. Grimwood
Stanford University
I. V. Grigoriev
DOE Joint Genome Institute
C. P. Kubicek
Technische Universitat Wien
D. Martinez
Los Alamos National Laboratory
University of New Mexico
N. N. M. E. Van Peij
DSM Biotechnology Center
J. A. Roubos
DSM Biotechnology Center
Jens B Nielsen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
S. E. Baker
Pacific Northwest National Laboratory
Genome Research
1088-9051 (ISSN) 1549-5469 (eISSN)
Vol. 21 6 885-897Drivkrafter
Hållbar utveckling
Styrkeområden
Energi
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier (SSIF 2011)
Genetik
DOI
10.1101/gr.112169.110