Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains
Artikel i vetenskaplig tidskrift, 2010
The field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to generate such a data set for a very large number of cellular components in the yeast Saccharomyces cerevisiae, a widely used model organism that is also used in the production of fuels, chemicals, food ingredients and pharmaceuticals. With the current focus on biofuels and sustainability, there is much interest in harnessing this species as a general cell factory. In this study, we characterized two yeast strains, under two standard growth conditions. We ensured the high quality of the experimental data by evaluating a wide range of sampling and analytical techniques. Here we show significant differences in the maximum specific growth rate and biomass yield between the two strains. On the basis of the integrated analysis of the high-throughput data, we hypothesize that differences in phenotype are due to differences in protein metabolism.
extraction
F-75700
chemostat cultures
-8093 Zurich
glycolytic-enzymes
genome
fermentative capacity
[Sherman
UMR 5800
metabolomics
network
minimum information
Switzerland.
David] CNRS
quantitative-analysis
saccharomyces-cerevisiae strains
Lab Bordelais Rech Informat
Författare
A.B. Canelas
Delft University of Technology
N. Harrison
University of Cambridge
A. Fazio
Danmarks Tekniske Universitet
Jie Zhang
Chalmers, Kemi- och bioteknik, Livsvetenskaper
J. P. Pitkanen
VTT Technical Research Centre of Finland
J. van den Brink
Delft University of Technology
B.M. Bakker
Vrije Universiteit Amsterdam
L. Bogner
Universitat Stuttgart
J. Bouwman
Vrije Universiteit Amsterdam
J. I. Castrillo
University of Cambridge
A. Cankorur
Bogazici Universitesi
Pramote Chumnanpuen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
P. Daran-Lapujade
Delft University of Technology
D. Dikicioglu
Bogazici Universitesi
K. Van Eunen
Vrije Universiteit Amsterdam
J. C. Ewald
ETH Zurich
J.J. Heijnen
Delft University of Technology
B. Kirdar
Bogazici Universitesi
I. Mattila
VTT Technical Research Centre of Finland
F.I. Mensonides
Vrije Universiteit Amsterdam
A. Niebel
Universitat Stuttgart
M. Penttila
VTT Technical Research Centre of Finland
J. Pronk
Delft University of Technology
M. Reuss
Universitat Stuttgart
L. Salusjarvi
VTT Technical Research Centre of Finland
Uwe H. Sauer
ETH Zurich
D. Sherman
CNRS Centre National de la Recherche Scientifique
M. Siemann-Herzberg
Universitat Stuttgart
H.V. Westerhoff
Vrije Universiteit Amsterdam
J.H. de Winde
Delft University of Technology
Dina Petranovic Nielsen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
S. G. Oliver
University of Cambridge
C. T. Workman
Danmarks Tekniske Universitet
N. Zamboni
ETH Zurich
Jens B Nielsen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
Nature Communications
2041-1723 (ISSN)
Vol. 1 9Styrkeområden
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier (SSIF 2011)
Kemi
DOI
10.1038/ncomms1150