Proteomic Analysis of Plasma Membrane Vesicles
Artikel i vetenskaplig tidskrift, 2009
(Figure Presented) A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flow-cell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
membrane proteins
microparticles
proteins
membrane isolation
vesicles
plasma membranes
cells
proteomics
fractionation
secretory vesicles
immunoglobulin-e
lipids
receptor
vesiculation
integration
Författare
Brigitte Bauer
Chalmers University of Technology
M. Davidson
Nanoxis AB
Owe Orwar
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
Angewandte Chemie - International Edition
1433-7851 (ISSN) 1521-3773 (eISSN)
Vol. 48 9 1656-1659Ämneskategorier (SSIF 2011)
Fysikalisk kemi
Kemi
DOI
10.1002/anie.200803898