Metabolic engineering of photorespiration
Book chapter, 2017
The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements.
Arabidopsis transformation
Agrobacterium tumefaciens
PCR amplification
Binary vectors
Author
Martin Engqvist
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
V.G. Maurino
Heinrich Heine Universitat
Methods in Molecular Biology
10643745 (ISSN)
137-155Subject Categories (SSIF 2011)
Microbiology
DOI
10.1007/978-1-4939-7225-8_10