Guanine Can Direct Binding Specificity of Ru–dipyridophenazine (dppz) Complexes to DNA through Steric Effects
Journal article, 2017
X-ray crystal structures of three ?-[Ru(L)2dppz]2+ complexes (dppz=dipyridophenazine; L=1,10-phenanthroline (phen), 2,2?-bipyridine (bpy)) bound to d((5BrC)GGC/GCCG) showed the compounds intercalated at a 5?-CG-3? step. The compounds bind through canted intercalation, with the binding angle determined by the guanine NH2 group, in contrast to symmetrical intercalation previously observed at 5?-TA-3? sites. This result suggests that canted intercalation is preferred at 5?-CG-3? sites even though the site itself is symmetrical, and we hypothesise that symmetrical intercalation in a 5?-CG-3? step could give rise to a longer luminescence lifetime than canted intercalation.
nucleic acids
DNA
structural biology
DNA structures
Author
James P. Hall
University of Reading
Diamond Light Source
Sarah P. Gurung
Diamond Light Source
University of Reading
Jessica Henle
University of Reading
Patrick Poidl
University of Reading
Johanna Andersson
Chalmers, Chemistry and Chemical Engineering, Applied Chemistry
Per Lincoln
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Graeme Winter
Diamond Light Source
Thomas Sorensen
Diamond Light Source
David J. Cardin
University of Reading
John A. Brazier
University of Reading
Christine J. Cardin
University of Reading
Chemistry - A European Journal
0947-6539 (ISSN) 1521-3765 (eISSN)
Vol. 23 21 4981-4985Driving Forces
Sustainable development
Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories (SSIF 2011)
Chemical Sciences
DOI
10.1002/chem.201605508