The direction of human mesenchymal stem cells into the chondrogenic lineage is influenced by the features of hydrogel carriers
Journal article, 2017
Low back pain is a major public health issue in the Western world, one main cause is believed to be intervertebral disc (IVD) degeneration. To halt/diminish IVD degeneration, cell therapy using different biomaterials e.g. hydrogels as cell carriers has been suggested. In this study, two different hydrogels were examined (in vitro) as potential cell carriers for human mesenchymal stem cells (hMSCs) intended for IVD transplantation. The aim was to investigate cell- survival and chondrogenic differentiation of hMSCs when cultured in hydrogels Puramatrix((R)) or Hydromatrix((R)) and potential effects of stimulation with growth hormone (GH). hMSCs/hydrogel cultures were investigated for cell-viability, attachment, gene expressionof chondrogenic markers SOX9, COL2A1, ACAN and accumulation of extracellular matrix (ECM). In both hydrogel types, hMSCs were viable for 28 days, expressed integrin beta 1 which indicates adhesion of hMSCs. Differentiation was observed into chondrocyte-like cells, in a higher extent in hMSCs/Hydromatrix((R)) cultures when compared to hMSCs/Puramatrix ((R)) hydrogel cultures. Gene expression analyses of chondrogenic markers verified results. hMSCs/hydrogel cultures stimulated with GH displayed no significant effects on chondrogenesis. In conclusion, both hydrogels, especially Hydromatrix((R)) was demonstrated as a promising cell carrier in vitro for hMSCs, when directed into chondrogenesis. This knowledge could be useful in biological approaches for regeneration of degenerated human IVDs.
in-vitro
p73
intervertebral disc degeneration
Hydrogels
repair
applications
Disc degeneration
Cell carrier
vivo
histochemie
low-back-pain
v4
model
1964
Cell Biology
Human mesenchymal stem cells
Anatomy & Morphology
growth-factors
tissue-engineering
therapy
Intervertebral disc
transplantation
ott j. e.
Author
A. Hansson
Chalmers University of Technology
Sahlgrenska Academy
Anna Wenger
Chalmers, Biology and Biological Engineering
H. B. Henriksson
Sahlgrenska Academy
Sahlgrenska Universitetssjukhuset
S. Li
Goteborgs universitet, Institutionen for biomedicin
B. R. Johansson
Goteborgs universitet, Institutionen for biomedicin
H. Brisby
Sahlgrenska Universitetssjukhuset
Sahlgrenska Academy
Tissue and Cell
0040-8166 (ISSN)
Vol. 49 1 35-44Subject Categories (SSIF 2011)
Cell and Molecular Biology
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1016/j.tice.2016.12.004