A lipid-based passivation scheme for nanofluidics

Joachim Fritzsche; F. Persson; K. U. Mir; M. Modesti; Fredrik Westerlund; J. O. Tegenfeldt

A lipid-based passivation scheme for nanofluidics
Paper in proceeding, 2012

Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.

Single molecule level

Genomic DNA

Bovine serum

Lipid bilayer

Lipid bilayers

Nano channels

Enzymatic Degradation

Molecules

Proteins

DNA

Single molecules

Single molecule

Passivation

Protein-DNA interactions

Nanofluidics

DNA cleavage

Author

Joachim Fritzsche

University of Gothenburg

Other publications Research

F. Persson

Uppsala Universitet

Goteborgs Universitet

K. U. Mir

Wellcome Trust Centre for Human Genetics

M. Modesti

Aix Marseille Universite

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Other publications Research

J. O. Tegenfeldt

University of Gothenburg

16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012; Okinawa; Japan; 28 October 2012 through 1 November 2012

1876-1878
9780979806452 (ISBN)

Subject Categories (SSIF 2011)

Physical Chemistry

Physical Sciences

ISBN

9780979806452

More information

Created

10/8/2017

A lipid-based passivation scheme for nanofluidics Paper in proceeding, 2012