Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels
Paper in proceeding, 2010

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Emission enhancement

Single molecule

Nanofluidics

Optical phenomena

Fluorescence enhancement

Fluorescence microscopy

Molecules

Quantitative fluorescence microscopy

Enhancement effects

DNA

Emission intensity

Single DNA molecules

Author

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

University of Gothenburg

Other publications Research

Fredrik Persson

University of Gothenburg

Other publications Research

A. Kristensen

Danmarks Tekniske Universitet

J. O. Tegenfeldt

University of Gothenburg

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010

Vol. 1 578-580
9781618390622 (ISBN)

Subject Categories (SSIF 2011)

Physical Sciences

ISBN

9781618390622

More information

Created

10/7/2017

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