Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance
Paper in proceeding, 2015

The use, and overuse, of antibiotics has during the last decade led to a dramatic increase in antibiotic resistance and there is a crying need for novel methods for fast identification of antibiotic resistance genes. We here demonstrate how our previously developed assay for optical mapping of DNA in nanochannels can be used for characterization of resistance genes located on plasmid DNA from bacteria. The assay requires extremely small sample volumes and does neither rely on PCR, nor culturing of bacteria, which greatly reduces the time for analysis.

Optical mapping

Nanofluidics

Plasmid DNA

Antibiotic resistance

Author

Lena Nyberg

Chalmers, Biology and Biological Engineering, Chemical Biology

Other publications Research

Gustav Emilsson

Chalmers, Applied Physics, Bionanophotonics

Other publications Research

A. Nilsson

Lunds Universitet

E. Lagerstedt

Lunds Universitet

C. Noble

Lunds Universitet

Liselott Svensson-Stadler

University of Gothenburg

N. Karami

University of Gothenburg

Fei Sjöberg

University of Gothenburg

Edward R.B. Moore

University of Gothenburg

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Other publications Research

Erik Kristiansson

University of Gothenburg

Other publications Research

T. Ambjornsson

Lunds Universitet

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Other publications Research

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

1045-1047
978-09-79-80647-6 (ISBN)

Areas of Advance

Nanoscience and Nanotechnology

Life Science Engineering (2010-2018)

Subject Categories (SSIF 2011)

Biochemistry and Molecular Biology

ISBN

978-09-79-80647-6

More information

Created

10/8/2017

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