Fast size-determination of intact bacterial plasmids using nanofluidic channels
Journal article, 2015
We demonstrate how nanofluidic channels can be used as a tool to rapidly determine the number and sizes of plasmids in bacterial isolates. Each step can be automated at low cost, opening up opportunities for general use in microbiology labs.
Author
Karolin Frykholm
Chalmers, Biology and Biological Engineering, Chemical Biology
Lena Nyberg
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
E. Lagerstedt
Lunds Universitet
C. Noble
Lunds Universitet
Joachim Fritzsche
Chalmers, Applied Physics, Chemical Physics
N. Karami
Sahlgrenska Universitetssjukhuset
T. Ambjornsson
Lunds Universitet
L. Sandegren
Uppsala Universitet
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
Lab on a Chip - Miniaturisation for Chemistry and Biology
1473-0197 (ISSN) 1473-0189 (eISSN)
Vol. 15 13 2739-2743Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories (SSIF 2011)
Biochemistry and Molecular Biology
DOI
10.1039/c5lc00378d
PubMed
25997119