Bridging omics technologies with synthetic biology in yeast industrial biotechnology
Book chapter, 2013
Metabolic engineering, defined as the practice of manipulating cells' genetic and regulatory processes for improving cellular performance, has been recently integrating systems and synthetic biology with other technologies, e.g. molecular biology, physiology, biochemistry, analysis science, bioinformatics and biochemical engineering. The aim is to surmount cells' performance-related limitations, e.g. metabolic limitation of cellular processes, network rigidity and global regulation, in a comprehensive, rational and high-throughput manner. Using this multi-level/integrated approach, the (re)design and (re)construction of microbial systems for the development of novel products with significant impact on current global problems, e.g. depletion of energy resources and global warming, is becoming a reality. In the last two decades, technological platforms for systems biology, e.g. genomics, transcriptomics, proteomics, metabolomics and fluxomics, and synthetic biology approaches, e.g. synthetic biological parts, devices and systems, have been implemented and efficiently used as tools for metabolic engineering of high-value bioproducts. One successful story on how metabolic engineering has been used to enhance the synthesis of microbial-based molecules is the production of biofuels. In this review, synthetic and systems biology technologies for yeast metabolic engineering will be described in detail. In addition, two case studies related with biofuel production (ethanol and 1-butanol) in yeast S. cerevisiae will be presented.
Systems biology
Biofuels
Synthetic biology
Metabolic engineering
Antisense RNA
Data mining
Industrial biotechnology
Genomics
Butanol
Riboswitches
Promoter binding proteins
Genome-scale metabolic models
Ribozymes
Ethanol
Metabolomics
Transcriptomics
Synthetic pathway
Riboregulators
Promoter libraries
Illumina sequencing
Targeted gene expression
Renewable resources
Proteomics
Promoter shuffling
Multi-gene assembly
High-value bioproducts
Yeast
Protein scaffolds
Microarrays
RNAseq
Fluxomics
Saccharomyces cerevisiae
Author
Antonio Roldao
Chalmers, Chemical and Biological Engineering, Life Sciences
Il-Kwon Kim
Chalmers, Chemical and Biological Engineering, Life Sciences
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
Systems Metabolic Engineering
271-327
978-94-007-4533-9 (ISBN)
Subject Categories (SSIF 2011)
Biological Sciences
DOI
10.1007/978-94-007-4534-6_9
ISBN
978-94-007-4533-9