Detection of peptide-based nanoparticles in blood plasma by ELISA
Journal article, 2015
Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
Author
G.H. Bode
Maastricht University
K.E. Pickl
Joanneum Research Forschungsgesellschaft mbH
M. Sanchez-Purrà
Institut Quimic de Sarria
B. Albaiges
Institut Quimic de Sarria
S. Borrós
Universitat Ramon Llull
Institut Quimic de Sarria
A.J.G. Pötgens
AplaGen GmbH
C. Schmitz
Ludwig-Maximilians-Universitat Munchen
F.M. Sinner
Medizinische Universitat Graz
Joanneum Research Forschungsgesellschaft mbH
M. Losen
Maastricht University
H.W.M. Steinbusch
Maastricht University
H.G. Frank
AplaGen GmbH
Ludwig-Maximilians-Universitat Munchen
P. Martinez-Martinez
Maastricht University
K. Wagemann
Society for Chemical Engineering and Biotechnology
H.A. Klok
Ecole Polytechnique Federale de Lausanne
R.E. Unger
Johannes Gutenberg Universitat Mainz
T. Pieber
Medizinische Universitat Graz
A. Cesàro
Universita degli Studi di Trieste
J. F. J. Engbersen
University of Twente
Bengt Herbert Kasemo
Chalmers, Applied Physics, Chemical Physics
M. Moeller
Deutsches Wollforschungsinstitut
R. Korenstein
Tel Aviv University
C. Grandfils
Universite de Liege
A. Bernkop-Schnuerch
University of Innsbruck
C. Kiparissides
Aristotle University of Thessaloniki
S. Slomkowski
Institute - Center for Molecular and Macromolecular Studies of the Polish Academy of Sciences
P. Venturini
National Institute of Chemistry Ljubljana
C.M. Paleos
Demokritos National Centre for Scientific Research
B. Podobnik
Lek Pharmaceuticals
P. Borm
Diagnostics BV
MagnaMedics Diagnostics BV
E.C.A. Van Winden
J. Groll
Deutsches Wollforschungsinstitut
B. Zassler
ThioMatrix GmbH
G. Gregoriadis
Lipoxen Plc
P.K. Nielsen
Novo Nordisk AS
A. Elouahabi
GlaxoSmithKline
PLoS ONE
1932-6203 (ISSN)
Vol. 10 5 Art. no. e0126136- e0126136Subject Categories (SSIF 2011)
Analytical Chemistry
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
DOI
10.1371/journal.pone.0126136