Droplet based directed evolution of yeast cell factories doubles production of industrial enzymes
Paper in proceeding, 2013
We present a high throughput micro-droplet based method for directed evolution of yeast cell factories for improved production of industrial enzymes. The workflow includes a fluorescently activated droplet sorter which was found to accurately sort droplets with a false positive rate of 0.0002 at 300 Hz. The workflow was used to screen a library of α-amylase expressing yeast mutants. A candidate yeast strain with a more than twofold increase in α-amylase production was isolated from a single round of directed evolution.
Author
S. L. Sjostrom
The Royal Institute of Technology (KTH)
Y. Bai
The Royal Institute of Technology (KTH)
M. Huang
Chalmers University of Technology
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
H. N. Joensson
The Royal Institute of Technology (KTH)
H. A. Svahn
The Royal Institute of Technology (KTH)
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
1270-1272
978-163266624-6 (ISBN)
Subject Categories (SSIF 2011)
Industrial Biotechnology
ISBN
978-163266624-6