Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate
Journal article, 2014
Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed.
Author
Valeria Wallace-Salinas
Lunds Universitet
Lorenzo Signori
Universita degli Studi di Milano - Bicocca
Ying-Ying Li
Institute of Botany and Microbiology
Flanders Interuniversity Institute for Biotechnology
Magnus Ask
Chalmers, Chemical and Biological Engineering, Industrial biotechnology
Maurizio Bettiga
Chalmers, Chemical and Biological Engineering, Industrial biotechnology
Danilo Porro
Universita degli Studi di Milano - Bicocca
Johan Thevelein
Institute of Botany and Microbiology
Flanders Interuniversity Institute for Biotechnology
Paola Branduardi
Universita degli Studi di Milano - Bicocca
Maria Foulquie-Moreno
Institute of Botany and Microbiology
Flanders Interuniversity Institute for Biotechnology
Marie Francoise Gorwa-Grauslund
Lunds Universitet
AMB Express
2191-0855 (ISSN)
Vol. 4 1 56- 56Driving Forces
Sustainable development
Subject Categories (SSIF 2011)
Industrial Biotechnology
Biochemistry and Molecular Biology
Bioenergy
Areas of Advance
Energy
Life Science Engineering (2010-2018)
DOI
10.1186/s13568-014-0056-5