In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility
Journal article, 2014
There is an increased interest in secondary plant metabolites, such as polyphenols and carotenoids, due to their proposed health benefits. Much attention has focused on their bioavailability, a prerequisite for further physiological functions. As human studies are time consuming, costly, and restricted by ethical concerns, in vitro models for investigating the effects of digestion on these compounds have been developed and employed to predict their release from the food matrix, bioaccessibility, and assess changes in their profiles prior to absorption. Most typically, models simulate digestion in the oral cavity, the stomach, the small intestine, and, occasionally, the large intestine. A plethora of models have been reported, the choice mostly driven by the type of phytochemical studied, whether the purpose is screening or studying under close physiological conditions, and the availability of the model systems. Unfortunately, the diversity of model conditions has hampered the ability to compare results across different studies. For example, there is substantial variability in the time of digestion, concentrations of salts, enzymes, and bile acids used, pH, the inclusion of various digestion stages; and whether chosen conditions are static (with fixed concentrations of enzymes, bile salts, digesta, and so on) or dynamic (varying concentrations of these constituents). This review presents an overview of models that have been employed to study the digestion of both lipophilic and hydrophilic phytochemicals, comparing digestive conditions in vitro and in vivo and, finally, suggests a set of parameters for static models that resemble physiological conditions.
in vitro models
DYNAMIC GUT MODEL
V34
GREEN TEA CATECHINS
P197
BAQCHA.S
HUMAN INTESTINAL MICROBIOTA
WINE/GRAPE JUICE
ATLA-ALTERNATIVES TO LABORATORY ANIMALS
SIMULATED GASTROINTESTINAL DIGESTION
bioacessibility
CACO-2 CELLS
BETA-CAROTENE
LLER DD
P2248
gastrointestinal digestion
CELL-CULTURE MODEL
1995
polyphenols
phytochemicals
1968
AMERICAN JOURNAL OF CLINICAL NUTRITION
GASTRIC-ACID SECRETION
NEKUS M
Food Science & Technology
SWEET-POTATO
V2
LANCET
FLESHED
P12
RED
V23
1981
carotenoids
Author
Marie Alminger
Chalmers, Chemical and Biological Engineering, Life Sciences
A. M. Aura
T. Bohn
C. Dufour
S. N. El
A. Gomes
S. Karakaya
M. C. Martinez-Cuesta
G. J. McDougall
T. Requena
C. N. Santos
Gastroenterology V. P. Rriere F
British Journal Of Nutrition V. P. Rry Jl
Comprehensive Reviews in Food Science and Food Safety
1541-4337 (ISSN)
Vol. 13 4 413-436Subject Categories (SSIF 2011)
Food Science
Food Engineering
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1111/1541-4337.12081