Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties
Journal article, 2014
AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.
1989
SPORULATION
SPECIFICITY
RAUCH MA
V246
TRANSITION-STATE REGULATOR
P1615
eilly M
P1203
1995
MYCOBACTERIUM-TUBERCULOSIS
RAUCH MA
P6999
DOMAIN
RAUCH MA
TYROSINE
V177
P522
1995
PROMOTER
V8
V179
JOURNAL OF BACTERIOLOGY
RAUCH MA
APRE EXPRESSION
1989
PROTEIN-KINASES
TRANSCRIPTION
MOLECULAR MICROBIOLOGY
V3
P756
1997
JOURNAL OF BACTERIOLOGY
EMBO JOURNAL
MOLECULAR & GENERAL GENETICS
Author
A. Kobir
Microbiologie de l'Alimentation au Service de la Sante Humaine
S. Poncet
Microbiologie de l'Alimentation au Service de la Sante Humaine
V. Bidnenko
Microbiologie de l'Alimentation au Service de la Sante Humaine
O. Delumeau
Microbiologie de l'Alimentation au Service de la Sante Humaine
C. Jers
Microbiologie de l'Alimentation au Service de la Sante Humaine
S. Zouhir
LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063
R. Grenha
LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063
S. Nessler
Universite Paris-Sud XI
LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063
P. Noirot
Microbiologie de l'Alimentation au Service de la Sante Humaine
Ivan Mijakovic
Chalmers, Chemical and Biological Engineering, Life Sciences
Molecular Microbiology
0950-382X (ISSN) 1365-2958 (eISSN)
Vol. 92 5 1129-1141Subject Categories (SSIF 2011)
Biological Systematics
DOI
10.1111/mmi.12617