Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism
Journal article, 2013
Production of fuels and chemicals by industrial biotechnology requires efficient, safe and flexible cell factory platforms that can be used for production of a wide range of compounds. Here we developed a platform yeast cell factory for efficient provision of acetyl-CoA that serves as precursor metabolite for a wide range of industrially interesting products. We demonstrate that the platform cell factory can be used to improve the production of alpha-santalene, a plant sesquiterpene that can be used as a perfume by four-fold. This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products.
sequence
Saccharomyces cerevisiae
saccharomyces-cerevisiae
Cell factory
synthetase
fermentation
expression
growth-rate
alpha-Santalene
transcription
precursor
pyruvate-decarboxylase
glucose
Acetyl-CoA
Author
Yun Chen
Chalmers, Chemical and Biological Engineering, Life Sciences
L. Daviet
Firmenich SA
M. Schalk
Firmenich SA
Verena Siewers
Chalmers, Chemical and Biological Engineering, Life Sciences
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
Metabolic Engineering
1096-7176 (ISSN) 1096-7184 (eISSN)
Vol. 15 1 48-54Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)
European Commission (FP7) (EC/FP7/247013), 2010-01-01 -- 2014-12-31.
Driving Forces
Sustainable development
Subject Categories (SSIF 2011)
Biological Sciences
Areas of Advance
Energy
Life Science Engineering (2010-2018)
DOI
10.1016/j.ymben.2012.11.002