Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Journal article, 2012
Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for on/off experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.
photon distribution analysis
photoinduced electron-transfer
multiparameter fluorescence detection
alternating-laser
rna-polymerase-ii
resonance energy-transfer
FRET
nucleic acids
DNA structures
excitation
double-stranded dna
fluorescence spectroscopy
analysis
probability-distribution
fluorescent probes
single-molecule fret
nano-positioning system
Author
S. Preus
Kobenhavns Universitet
Marcus Wilhelmsson
Chalmers, Chemical and Biological Engineering, Physical Chemistry
ChemBioChem
1439-4227 (ISSN) 1439-7633 (eISSN)
Vol. 13 14 1990-2001Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories (SSIF 2011)
Physical Chemistry
Biochemistry and Molecular Biology
Biophysics
Chemical Sciences
DOI
10.1002/cbic.201200400