Bacterial cellulose modified with xyloglucan bearing the adhesion peptide RGD promotes endothelial cell adhesion and metabolism--a promising modification for vascular grafts.
Journal article, 2011
Today, biomaterials such as polytetrafluorethylene (ePTFE) are used clinically as prosthetic grafts for vascular surgery of large vessels (>5 mm). In small diameter vessels, however, their performance is poor due to early thrombosis. Bacterial-derived cellulose (BC) is a new promising material as a replacement for blood vessels. This material is highly biocompatible in vivo but shows poor cell adhesion. In the native blood vessel, the endothelium creates a smooth non-thrombogenic surface. In order to sustain cell adhesion, BC has to be modified. With a novel xyloglucan (XG) glycoconjugate method, it is possible to introduce the cell adhesion peptide RGD (Arg-Gly-Asp) onto bacterial cellulose. The advantage of the XG-technique is that it is an easy one-step procedure carried out in water and it does not weaken or alter the fiber structure of the hydrogel. In this study, BC was modified with XG and XGRGD to asses primary human vascular endothelial cell adhesion, proliferation, and metabolism as compared with unmodified BC. This XG-RGD-modification significantly increased cell adhesion and the metabolism of seeded primary endothelial cells as compared with unmodified BC whereas the proliferation rate was affected only to some extent. The introduction of an RGD-peptide to the BC surface further resulted in enhanced cell spreading with more pronounced stress fiber formation and mature phenotype. This makes BC together with the XG-method a promising material for synthetic grafts in vascular surgery and cardiovascular research.
drug effects
Cellulose
pharmacology
Staining and Labeling
chemistry
Glucans
drug effects
methods
Gluconacetobacter xylinus
Stress Fibers
drug effects
Phase-Contrast
cytology
Oligopeptides
Cell Adhesion
drug effects
Humans
Cell Movement
Cattle
metabolism
Animals
drug effects
pharmacology
Serum
Fluorescence
Cell Shape
Endothelial Cells
Xylans
Cell Proliferation
pharmacology
metabolism
drug effects
Vascular Grafting
Microscopy
pharmacology
Author
Helen Fink
University of Gothenburg
Lage Ahrenstedt
The Royal Institute of Technology (KTH)
Aase Katarina Bodin
Chalmers, Chemical and Biological Engineering, Polymer Technology
Harry Brumer
The Royal Institute of Technology (KTH)
Paul Gatenholm
Chalmers, Chemical and Biological Engineering, Polymer Technology
Alexandra Krettek
University of Gothenburg
Bo Risberg
University of Gothenburg
Journal of tissue engineering and regenerative medicine
1932-7005 (eISSN)
Vol. 5 6 454-63Subject Categories (SSIF 2011)
MEDICAL AND HEALTH SCIENCES
DOI
10.1002/term.334
PubMed
21604383