Comparative Metabolic Capabilities for Micrococcus luteus NCTC 2665, the "Fleming" Strain, and Actinobacteria
Journal article, 2011
Putative gene predictions of the Gram positive actinobacteria Micrococcus luteus (NCTC 2665, "Fleming strain") was used to construct a genome scale reconstruction of the metabolic network for this organism. The metabolic network comprises 586 reactions and 551 metabolites, and accounts for 21% of the genes in the genome. The reconstruction was based on the annotated genome and available biochemical information. M. luteus has one of the smallest genomes of actinobacteria with a circular chromosome of 2,501,097 base pairs and a GC content of 73%. The metabolic pathways required for biomass production in silico were determined based on earlier models of actinobacteria. The in silico network is used for metabolic comparison of M. luteus with other actinomycetes, and hence provides useful information for possible future biotechnological exploitation of this organism, e. g., for production of biofuels.
metabolic network
scale
analysis
complete genome sequence
Micrococcus luteus
protein families
in silico biomass
genus micrococcus
cohn 1872
streptomyces-coelicolor a3(2)
mycobacterium-tuberculosis
biosynthesis
yield
database
actinobacteria
annotation
Author
J. S. Rokem
Hebrew University-Hadassah Medical School
Wanwipa Vongsangnak
Chalmers, Chemical and Biological Engineering, Life Sciences
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
Biotechnology and Bioengineering
0006-3592 (ISSN) 1097-0290 (eISSN)
Vol. 108 11 2770-2775Subject Categories (SSIF 2011)
Industrial Biotechnology
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1002/bit.23212