The Influence of Microgravity on Invasive Growth in Saccharomyces cerevisiae
Journal article, 2011
This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Sigma 1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Sigma 1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Sigma 1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.
candida-albicans
immune-system
cell-cell
Budding
international-space-station
shear modeled microgravity
Microgravity
Adhesion
biofilm formation
stress-response
yeast
Saccharomyces cerevisiae
gene-expression
Invasive growth
Proteomics
escherichia-coli
Author
S. E. Van Mulders
KU Leuven
C. Stassen
Universiteit Gent
L. Daenen
KU Leuven
B. Devreese
Universiteit Gent
Verena Siewers
Chalmers, Chemical and Biological Engineering, Life Sciences
R. G. E. van Eijsden
Flanders Interuniversity Institute for Biotechnology
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
F. R. Delvaux
KU Leuven
R. G. Willaert
Vrije Universiteit Brussel
Astrobiology
1531-1074 (ISSN)
Vol. 11 1 45-55Subject Categories (SSIF 2011)
Biological Sciences
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1089/ast.2010.0518