Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons
Journal article, 2010
We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup containing 8.4 mg of beta-carotene and 9 mg of lycopene. After 5 h, the subjects consumed a second meal without carotenoids. Blood samples were collected at basal time and every hour for 9 h. Chylomicrons were isolated from serum samples and used for both carotenoid quantification and HepG2 stimulation. Carotenoid in chylomicrons followed an inter-individual and bimodal carotenoid response. We demonstrated the antioxidant effect of postprandial chylomicrons in HepG2 at the time of maximum carotenoid concentration of chylomicrons with respect to basal time. This cell-based assay seems to be a useful method to evaluate the antioxidant effect of fruit and vegetable products in a biological system.
lycopene
postprandial
chylomicron
beta-carotene
metabolism
salmonella-typhimurium
HepG2 cells
remnants
hepg2 cells
dietary
plasma
tomato lycopene
oxidative stress
bioavailability
vegetable and fruit food
Reactive oxygen species
beta-carotene
Author
M. Sanchez-Campillo
Universidad de Murcia
F. Perez-Llamas
Universidad de Murcia
D. Gonzalez-Silvera
Universidad de Murcia
R. Martinez-Tomas
Universidad de Murcia
M. I. Burgos
Hospital Virgen de la Arrixaca
Anna Wellner
Chalmers, Chemical and Biological Engineering, Life Sciences
F. Aviles
Hospital Virgen de la Arrixaca
S. Parra
Hospital Virgen de la Arrixaca
L Bialek
Unilever R&D Vlaardingen
Marie Alminger
Chalmers, Chemical and Biological Engineering, Life Sciences
E. Larque
Universidad de Murcia
Journal of Agricultural and Food Chemistry
0021-8561 (ISSN) 1520-5118 (eISSN)
Vol. 58 20 10864-10868Subject Categories (SSIF 2011)
Food Science
Chemical Sciences
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1021/jf102627g