Intravital fluorescent microscopic evaluation of bacterial cellulose as scaffold for vascular grafts.
Journal article, 2010
Although commonly used synthetic vascular grafts perform satisfactorily in large caliber blood vessels, they are prone to thrombosis in small diameter vessels. Therefore, small vessels might benefit from tissue engineered vascular grafts. This study evaluated bacterial cellulose (BC) as a potential biomaterial for biosynthetic blood vessels. We implanted the dorsal skinfold chambers in three groups of Syrian golden hamsters with BC (experimental group), polyglycolic acid, or expanded polytetrafluorethylene (control groups). Following implantation, we used intravital fluorescence microscopy, histology, and immunohistochemistry to analyze the biocompatibility, neovascularization, and incorporation of each material over a time period of 2 weeks. Biocompatibility was good in all groups, as indicated by the absence of leukocyte activation upon implantation. All groups displayed angiogenic response in the host tissue, but that response was highest in the polyglycolic acid group. Histology revealed vascularized granulation tissue surrounding all three biomaterials, with many proliferating cells and a lack of apoptotic cell death 2 weeks after implantation. In conclusion, BC offers good biocompatibility and material incorporation compared with commonly used materials in vascular surgery. Thus, BC represents a promising new biomaterial for tissue engineering of vascular grafts.
Blood Vessel Prosthesis
drug effects
pathology
Acetobacter
Inflammation
Neovascularization
Immunohistochemistry
Cricetinae
drug effects
chemistry
Fluorescence
Mesocricetus
Leukocyte Rolling
Female
Tissue Scaffolds
pharmacology
drug effects
Microscopy
methods
drug effects
Animals
Hemodynamics
Physiologic
Cellulose
Materials Testing
physiology
drug effects
chemistry
Cell Adhesion
Microvessels
Author
Maricris Esguerra
University of Gothenburg
Helen Fink
University of Gothenburg
Matthias W Laschke
Universitatsklinikum des Saarlandes Medizinische Fakultat der Universitat des Saarlandes
Anders Jeppsson
University of Gothenburg
Dick Delbro
University of Gothenburg
Paul Gatenholm
Chalmers, Chemical and Biological Engineering, Polymer Technology
Michael D Menger
Universitatsklinikum des Saarlandes Medizinische Fakultat der Universitat des Saarlandes
Bo Risberg
University of Gothenburg
Journal of biomedical materials research. Part A
1552-4965 (eISSN)
Vol. 93 1 140-9Subject Categories (SSIF 2011)
Surgery
MEDICAL AND HEALTH SCIENCES
DOI
10.1002/jbm.a.32516
PubMed
19536832