Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae
Journal article, 2009
The construction of mitotically stable yeast strains for heterologous gene or pathway expression often requires chromosomal integration. However, transcription levels vary between different chromosome regions. We therefore characterized 20 different integration sites of the Sacchromyces cerevisiae genome by inserting lacZ as a reporter gene under the control of two different promoters and determining expression levels through enzyme activity measurement. An up to 8.7-fold difference was detected between the sites conferring lowest and highest expression, respectively. This opens the opportunity for modulating gene expression levels while retaining promoter and culture conditions. Copyright (C) 2009 John Wiley & Sons, Ltd.
yeast
pathway
specificity
Saccharomyces cerevisiae
decarboxylase
beta-carotene
sequence
structural gene
lacZ
solo LTRs
reporter gene
escherichia-coli
precursor
genome
Author
D. B. Flagfeldt
V. Siewers
L. Huang
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
Yeast
0749-503X (ISSN) 1097-0061 (eISSN)
Vol. 26 10 545-551Subject Categories (SSIF 2011)
Industrial Biotechnology
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1002/yea.1705