Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing
Journal article, 2008
Pucker up! A novel approach enabling direct measurements of biomolecular transfer across lipid bilayer membranes, using surface plasmon resonance, is demonstrated. The figure shows the transfer of sucrose (S) through melittin pores formed in surface-attached liposomes. By measuring the shift in refractive index in the volume enclosed by the liposomes, the sucrose transfer can be time-resolved and quantified.
CHOLESTEROL
melittin
LIPID VESICLES
MELITTIN
DNA
PLASMON RESONANCE SENSORS
SPR
liposomes
DRUG DISCOVERY
transport
surface plasmon resonance
membranes
Author
Magnus Brändén
Chalmers, Applied Physics, Biological Physics
S. Dahlin
Stena Centre 1B
Fredrik Höök
Chalmers, Applied Physics, Biological Physics
ChemPhysChem
1439-4235 (ISSN) 1439-7641 (eISSN)
Vol. 9 17 2480-2485Subject Categories (SSIF 2011)
Other Engineering and Technologies
DOI
10.1002/cphc.200800614